atp chemical (Millipore)
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atp chemical
Atp Chemical, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atp+chemical/atp+chemical/pm40631926-890-11-12
Average 90 stars, based on 1 article reviews
Atp Chemical, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atp+chemical/atp+chemical/pm40631926-890-11-12
Average 90 stars, based on 1 article reviews
atp chemical - by Bioz Stars,
2026-09
90/100 stars
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Produced:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. CRISPR:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Virus:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Recombinant:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Immunoprecipitation:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Modification:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Transfection:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. DNA Extraction:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Lysis:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Extraction:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Protease Inhibitor:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Reverse Transcription:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. SYBR Green Assay:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Polymerase Chain Reaction:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Software:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Standard Deviation:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Sequencing:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Cell Culture:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Staining:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. SDS Page:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Cryo-EM Sample Prep:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. High Performance Liquid Chromatography:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Expressing:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Purification:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Incubation:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Electrochemiluminescence:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Control:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. In Vivo:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Enzyme-linked Immunosorbent Assay:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Mutagenesis:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. Plasmid Preparation:Article Title: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-human b2-microglobulin (2M2)-APC BioLegend Cat#316312; RRID_AB_10641281 Monoclonal ANTI-FLAG M2 antibody produced in Article Title: The role of heparan sulfate in enhancing the chemotherapeutic response in triple-negative breast cancer. Article Snippet: The following drugs and chemicals were used: Article Title: Crystalline forms of a phosphoinositide 3-kinase (PI3K) inhibitor Article Snippet: ATP, MgCl2, DTT, EDTA, MOPS and Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP Article Snippet: The following drugs and chemicals were used: Article Title: FDA-approved disulfiram inhibits the NLRP3 inflammasome by regulating NLRP3 palmitoylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Flag GenScript Cat# A00187; RRID:AB_1720813 Mouse monoclonal anti-NLRP3(Cryo-2) Adipogen Cat# AG-20B-0014; RRID:AB_2490202 Rabbit monoclonal anti-NEK7 Abcam Cat# ab133514; RRID:AB_2877625 Rabbit monoclonal anti-GSDMD Abcam Cat# ab209845; RRID:AB_2783550 Mouse monoclonal IgG2b isotype control Cell signaling Cat# 53484; RRID:AB_2799435 Rat polyclonal anti-ASC Dr. Gabriel Núñez N/A Rabbit polyclonal anti-Caspase-1 Dr. Gabriel Núñez N/A THETM beta Actin Antibody [HRP] GenScript Cat# A00730; RRID:AB_914100 Bacterial and virus strains Salmonella Typhimurium Strain 14028 Dr. Denise Monack N/A Chemicals, peptides, and recombinant proteins High-capacity streptavidin agarose resin ThermoFisher Cat# 20359 Protein G beads GenScript Cat# L00209 LPS (Escherichia coli 055:B5, Ultrapure) InvivoGen Cat# tlrl-pb5lps LPS (Escherichia coli O111:B4, for in vivo expt) Article Title: Discovery of AZD4144, a Selective and Potent NLRP3 Inhibitor for the Treatment of Inflammatory Diseases. Article Snippet: Following 30− 40 min incubation, cells were triggered with 2 mM Article Title: Post-symptomatic NLRP3 inhibition rescues cognitive impairment and mitigates amyloid and tau driven neurodegeneration. Article Snippet: Following drug incubation, Article Title: Structural insights into binding-site access and ligand recognition by human ABCB1. Article Snippet: ABCB1 is a broad-spectrum efflux pump central to cellular drug handling and multidrug resistance in humans.. However, how it is able to recognize and transport a wide range of diverse substrates remains poorly understood.. Here we present cryo-EM structures of lipid-embedded human ABCB1 in conformationally distinct apo-, substrate-bound, inhibitor-bound, and nucleotide-trapped states at 3.4–3.9 Å resolution, in the absence of stabilizing antibodies or mutations. |
